Effects of exosomes derived from canine umbilical cord mesenchymal stem cells on proliferation, migration and apoptosis of vascular endothelial cells

doi:10.3969/j.issn.2095-4344.1808

Abstract

Abstract:

BACKGROUND: Recent studies have found that wound healing in dogs can be expedited by umbilical cord mesenchymal stem cell-derived exosomes (UC-MSC-Exo). Angiogenesis is an important process of wound healing, but the mechanism of UC-MSC-Exo on angiogenesis is yet unclear.
OBJECTIVE: To investigate the effects of UC-MSC-Exo on the proliferation, migration and apoptosis of vascular endothelial cells.
METHODS: Canine UC-MSC-Exo was isolated by low temperature ultracentrifugation. Canine vascular endothelial cells were isolated by mechanical exfoliation, digested by collagenase type I, and then identified by cell surface markers and differentiation characteristics. The effects of canine UC-MSC-Exo on proliferation, migration and apoptosis of vascular endothelial cells were determined by cell counting kit-8 method, wound healing test and Annexin V flow cytometry assay. High-throughput sequencing was used to detect the expression of miRNA in canine UC-MSC-Exo and to explore its possible mechanism.
RESULTS AND CONCLUSION: This study successfully established a method for isolation and culture of canine arterial endothelial cells with the “paving stone” morphology and positive expression of CD34 and CD31 surface proteins. The canine UC-MSC-Exo could significantly promote the proliferation and migration of vascular endothelial cells and inhibit cell apoptosis. And the mechanism might be related to the miRNAs enriched.

Key words: umbilical cord mesenchymal stem cells, exosomes, vascular endothelial cells, cell proliferation, cell migration, apoptosis, high-throughput sequencing, miRNA, Natural Science Foundation of Guangdong Province

CLC Number:

Zhan Xiaoshu, Luo Huina, Luo Dongzhang, Chen Shengfeng, Wang Bingyun, Bai Yinshan, Chen Zhisheng, Liu Canying, Ji Huiqin. Effects of exosomes derived from canine umbilical cord mesenchymal stem cells on proliferation, migration and apoptosis of vascular endothelial cells[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(29): 4637-4643.

Figures/Tables 3

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